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Question 1 of 30

1. Question
What is CRISPR?
- Sequences derived from viral DNA fragments
- A gene-editing tool
- A bacterial defence mechanism
- All of the answers
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Question 2 of 30

2. Question
Which component(s) is/are involved in CRISPR?
- Cas9 enzyme
- DNA
- sgRNA
- All of the answers
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Question 3 of 30

3. Question
The enzymatic activity of the Cas9 protein can be compared to:
- An anchor
- Scissors
- Glue
- Stapler
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Question 4 of 30

4. Question
Where does gene editing occur?
- Anywhere in the genome, depending on the sgRNA used
- Anywhere in the genome, as long as the sgRNA binds upstream of a PAM sequence
- Anywhere in the genome, as long as the sgRNA binds downstream of a PAM sequence
- Only in exons
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Question 5 of 30

5. Question
Which applications have been developed using CRISPR?
- Gene knock-in to create fusion proteins
- Transcriptional activation or inhibition of a gene of interest
- Chemically inducible gene editing
- Manipulation of the 3D genome structure within the nucleus
- All of the answers
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Question 6 of 30

6. Question
Which cellular DNA repair mechanism requires a DNA template?
- HDR
- NHEJ
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Question 7 of 30

7. Question
What is dCas9?
- Deactivated Cas9 with two point mutations at D10A and H840A
- DNA unwinding Cas9 with two point mutations at D10A and H840A
- DNA unwinding Cas9 with two point mutations at D840A and H10A
- Deactivated Cas9 with two point mutations at D840A and H10A
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Question 8 of 30

8. Question
What is not an ingredient of the Gibson assembly mix?
- Exonuclease
- Polymerase
- Ligase
- Endonuclease
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Question 9 of 30

9. Question
Main requisite for a target site corresponding to CRISPR/Cas9?
- Protospacer adjacent motif (PAM) sequence
- CAS/CPF
- gRNA oligonucleotides
- Fluorescent marker
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Question 10 of 30

10. Question
Which among the following is not a type of CRISPR induced techniques to develop a genetically edited crop?
- CRISPRi
- CRISPRa
- Virus interference
- Multiplex gene editing
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Question 11 of 30

11. Question
Cystic fibrosis is a type of:
- Autosomal recessive
- Autosomal dominant
- X-linked dominant
- X-linked recessive
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Question 12 of 30

12. Question
Cellular reprogramming is?
- Conversion of somatic cell into pluripotent stem cells
- Reestablishment of DNA methylation patterns in between generations of mammals
- Elimination of psychological trauma
- A single stem cell that has the capacity to differentiate into only one cell type
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Question 13 of 30

13. Question
Which of the following is an error-free repair mechanism
- SOS response
- HDR
- NHEJ
- None of the above
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Question 14 of 30

14. Question
Which of the country is the World has the highest usage of CRISPR as genome editing
tool?
- India
- China
- USA
- Korea
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Question 15 of 30

15. Question
Induced pluripotent stem cells can be directly generated from?
- Somatic cells
- Adult cells
- Germ cells
- Fibroblast cells
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Question 16 of 30

16. Question
Which one is the most widely used method for the detection of targeted mutations?
- The mismatch cleavage assay
- Target sequencing
- GUIDE-seq
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Question 17 of 30

17. Question
The ‘seed region’ of sgRNA refers to the:
- 1-5 base pair proximal to PAM site
- 10-12 base pairs proximal to PAM site
- The PAM site only
- The whole guide region of the sgRNA
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Question 18 of 30

18. Question
Cpf1 is a type of:
- Class 1 type II CRISPR-Cas system
- Class 2 type II CRISPR-Cas system
- Class 1 type V CRISPR-Cas system
- Class 2 type V CRISPR-Cas system
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Question 19 of 30

19. Question
Two Cp1-family proteins have been shown to perform efficient genome editing in human cells. Identify these two.
- FnCpf1 and PCpf1
- FnCpf1 andnLbCpf1
- AsCpf1 and LbCpf1
- AsCpf1 and PCpf1
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Question 20 of 30

20. Question
How does CRISPR-Cpf1 different from CRISPR-Cas9?
- Cpf1 enzyme is bigger than the standard spCas9
- Cpf1 system requires only a single RNA
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Question 21 of 30

21. Question
What is the other name of DSB repair pathway?
- RecBAD pathway
- RecBCD pathway
- RecABD pathway
- RecDCB pathway
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Question 22 of 30

22. Question
Which of these cannot be referred to as gene knockout?
- Elimination of a gene from the genome
- Adding multiple copies of the gene
- Breaking the coding sequence of gene of interest
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Question 23 of 30

23. Question
What is the desired length of sgRNA needed for cloning into plasmid?
- 4 nucleotides
- 300 nucleotides
- 20 nucleotides
- 2000 nucleotides
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Question 24 of 30

24. Question
Benefits of using a dual gRNA system in CRISPR/Cas9?
- Easy identification by target
- Removal of larger chunks of DNA
- No particular benefit
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Question 25 of 30

25. Question
RuvC cleaves which strand?
- Complimentary
- Non-complimentary
- Both the strands
- None
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Question 26 of 30

26. Question
What do type II CRISPR nuclease require?
- Cas9, crRNA, tracrRNA
- Cas9, crRNA, gRNA
- Only Cas9
- Only crRNA
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Question 27 of 30

27. Question
The best method to determine the genotype of the dominant parent is by crossing it with the hybrid. This cross is called:
- Back cross
- Test cross
- Selfing
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Question 28 of 30

28. Question
Which of the following genes undergo a functional loss that leads to HIV?
- CCR5
- CXCR4
- CD34+
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Question 29 of 30

29. Question
Haemophilia C is caused by the genetic mutation of which coagulation factor?
- X
- XII
- XI
- IV
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Question 30 of 30

30. Question
Haemophilia A is caused by the genetic mutation of which coagulation factor?
- IX
- XI
- V
- VIII
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