CRISPR Genome Editing Documentary

CRISPR Genome Editing Documentary

Breaking Boundaries: The Science of CRISPR Technology

CRISPR Genome Editing Documentary

Since the discovery of CRISPR/Cas9 system in 1987, its applications have expanded beyond the domains of gene-editing functionality and include epigenetic editing, gene regulation, chromatin engineering, editing of genome, transcriptome, and epigenome of animals, plants, and human. This, and the fact that stem cells can be cultured and grown in a lab, has resulted in achieving significant scientific breakthroughs. Discover how CRISPR is revolutionising the world in this documentary, from HIV, gene therapy, cancer, prime editing, to CRISPR-Chip. Learn more about the ethical concerns and future prospects of this amazing technology.

RELEASING DATE: 27/11/2021

AUDIENCE: open to everyone, to non-scientists too.

MODE: Online

PRICE: £19.99

MECHANISM OF
CRISPR-CAS SYSTEM

A CRISPR array is typically found within most bacterial and archaeal genomes. This array contains numerous unique protospacer sequences that have homology to specific foreign DNA. These protospacers have short palindromic repeat sequences between them. Below is an overview of the CRISPR/Cas9 system, an endogenous type II bacterial CRISPR system.

01
Upon spacer acquisition from invading DNA, CRISPR array is transcribed into pre-CRISPR RNA (pre-crRNA).
02
The trans-activating crRNA (tracrRNA), being complementary to the pre-crRNA forms an RNA duplex with the pre-crRNA. Within the RNA duplex, pre-crRNA matures to form crRNA by recruiting RNAse III. The RNA duplex containing crRNA with tracrRNA is also known as the single guide RNA (sgRNA).
03
The sgRNA complexes with Cas9 endonuclease to form the ribonucleoprotein complex that assists in target interception.
04
The ribonucleoprotein complex conducts target search and recognition based on the complementary base pairing of crRNA with the protospacer within the target DNA adjacent to the protospacer adjacent motif (PAM).
05
Upon binding of the ribonucleoprotein complex with the target DNA, the Cas9 facilitates the unwinding of the DNA double-helical strand at the target site and cleaves the double-stranded DNA at 3 base-pairs upstream of its PAM region.
06
After the double-stranded DNA cleavage, the ribonucleoprotein complex unbinds.

CRISPR/Cas is one of the ground-breaking discoveries in the biotechnological domain. The possibility of precise genome editing offers the promise of a cure, where conventional therapeutics has achieved limited success.

Recent developments in CRISPR technology enable its use for precise genome regulation and interrogation. Simultaneously it finds application as an imaging tool to track genomic loci in real time. Thus, the multiplex scope of engineering CRISPR/Cas9 has made it possible for scientists to relate this system for genome modifications in a wide range of organisms.